Measuring spectra in the InVision

1. Obtain ThorSpectra and sensor box from the shelf behind the InVision

2. Running ThorSpectra software on the Dell:
1. Turn on the Dell computer next to the Stemi
2. Log in with your account credentials
3. Once logged in, open the ThorSpectra shortcut on the desktop
3. Connecting the ThorSpectra:
1. Unwrap the cords around the spectrometer and plug the USB into one of the ports on the computer block
2. On the computer, click the “Connect Devices” button in the upper left hand of the screen

3. The spectrometer should connect automatically. If not, open the “Devices” menu next to “Connect Devices” to investigate
4. Attaching the sensor attachment to the spectrometer:
1. Carefully open the sensor attachment box
2. Align the end of the sensor cord with the sensor attachment and spin the silver threaded bolt while holding the sensor still to screw them together
❗

Do not spin the sensor attachment to screw it on


5. Collecting a background spectrum:
1. Set the “Integration Time” to 500.00 ms

2. Turn off the above-head lights in the room, turn off the LEDs in the InVision, and use the door of the InVision to block light from the computer screen
3. One person holds the sensor inside the InVision
4. The other person clicks “Update Dark” at the computer

5. “Dark Spectrum at 500 ms” will appear

6. Collecting traces:
1. Keep the “Integration Time” at 500.00 ms
2. Make sure there are 10 traces (labeled A-J). If not, add them

3. One person places the blue 10-slotted tray in the bottom arena holder, runs the sensor underneath the stage, and inserts it into the leftmost hole so that it is flush with the top

4. The person at the InVision signals “ready” to the person at the computer so that they can collect the trace
5. The person at the computer clicks on “Repeat” so that the ThorSpectra goes into data-collecting mode

6. Right-click on the first trace (A), hold the cursor over “Average and Fix”, and click on “10” once that menu pops up. The screen should fill in and average the light sensor output

7. The person at the computer signals “done” to the person at the InVision so that they can move on to the next slot
8. One at a time, moving left to right through all the slots, record each trace (A-J) at each spot in the tray (1-10)
9. Right-click the traces screen to autoscale (if needed)
7. Saving traces to OneDrive:
1. When done collecting all ten traces, hit “Stop” to turn off “Repeat” mode, go under “File”, and click on “Save All”

2. In the file pop-up menu, navigate to your personal OneDrive, click on your shortcut to “Wheeler Lab”, and go to “Data”
3. Click on the “project-miracidia_photosensation” or “project-mosquito-photosensation”
4. Within that folder, click on “data”, “raw”, and then the specific photosensation experiment folder like “red_gradient” (if there is one)
5. In the “raw” folder or the specific experiment folder, create a new folder with today’s date with the format “YYYYMMDD”
6. Save the file to that folder as a Text File with the name “all_tracesYYYYMMDD.txt” or “all_tracesYYYYMMDD(experiment type).txt” if multiple trace files are going into one date folder

7. For the format, set “Separate Columns by” to “Semicolon” and “New Line” to “Unix (LF)”, then click “OK” to save