Created
Jun 7, 2022 8:38 PM
Status
Complete
Category
SchistosomesSnailsRecipes
Schistosome and Snail Recipes
iBge Medium pH 7.2
- Filter sterilize (0.22 um filter) and store at 4C
To prepare cBge medium, add 10 mL of heat inactivated FBS and 1 mL of 100X pen/strep to 89 mL iBge.
Note: iBge is very easily contaminated
Note: Do not freeze cBge Medium
Note: Bge cells are sensitive to the quality of FBS. Several lots of FBS may have to be screened. It has been known that investigators using Bge cell lines have soon lost it due to the unsuitability of their FBS.
Hansen's Buffered Saline pH 7.5-7.7
| Reagent | Amount (per 1 L) |
|---|---|
NaCl | 2.8 g |
Sodium gluconate | 1.5 g |
KCl | 0.15 g |
Na2HPO4 | 0.07 g |
HCO3 | 0.05 g |
Glucose | 0.5 g |
Trehalose | 0.5 g |
Galactose | 0.5 g |
Sterile DI water | 1 L |
Chernin's Balanced Salt Solution (CBSS)
| Reagent | Amount (per 1 L) |
|---|---|
NaCl | 2.8 g |
KCl | 0.15 g |
Na2HPO4 | 0.07 g |
MgSO4 heptahydrate | 0.45 g |
CaCl dihydrate | 0.53 g |
NaHCO3 | 0.05 g |
Sterile DI water | 1 L |
- Filter sterilize (0.22 um filter) and store at 4C
- To make CBSS+, add 1 g each of glucose and trehalose
Trypsin Solution 0.05%
| Reagent | Amount |
|---|---|
Trypsin | 50 mg |
Hansen's buffered saline | 100 mL |
- Filter sterilize (0.22 um filter).
- Freeze in 10 mL aliquots.
- Warm before using.
Pond Water Stock Solution
| Reagent | Amount (per 1 L) |
|---|---|
CaCO3 | 12.5 g |
MgCO3 | 1.25 g |
NaCl | 1.25 g |
KCl | 0.25 g |
Sterile DI water | 1 L |
- Pond water stocks are prepared in small Nalgene containers.
- Shake the stock bottle well before diluting, as the salts may have settled on the bottom.
- Common working Pond Water dilutions:
- 100 mL Pond Water Stock → 30 gallons dH2O
- 33 mL Pond Water Stock → 10 gallons dH2O
- 16.5 mL Pond Water Stock → 5 gallons dH2O
- 12 mL Pond Water Stock → 10 liters dH2O